SULM – Schweizerische Union für Labormedizin | Union Suisse de Médecine de Laboratoire | Swiss Union of Laboratory Medicine

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L. Taborsky1 , P. Adam1 , E. Rihova2 , O. Sobek1

1Dpt Clin. Biochemistry, Hospital Na Homolce, Prague 5, 2Dpt of Ophtalmology, Charles University, Prague 2, Czech Republic.

Introduction:
Cytological proof of intraocular lymphomas is very difficult due to limited volume of samples obtained. Due to the developmental resemblance with cerebrospinal fluid, the cytological and biochemical parameters of intraocular media, i. e., vitreous humour and aqueous humour, are close to CSF and require the same style of handling of the samples.
Material and methods:
Using cytosedimentation method in sedimentation chamber, the cytological preparations were prepared and stained like CSF samples. The basic stain and Oil Red O stain were predominantly used.
Conclusions:
17 patients with suspection for primary intraocular lymphomas, expressed by ophtalmologist, the positivityof malignant cells was present. All of them were classified as non-Hodgkin lymphomas and the findings were photodocumented.
All the samples were collected during 8 years after introduction of routine handling protocol; and, till now, the group of 17 positive finding belongs to the largest groups of primary intraocular lymphomas described in literature.

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