SULM – Schweizerische Union für Labormedizin | Union Suisse de Médecine de Laboratoire | Swiss Union of Laboratory Medicine

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B Röthlisberger1 , E Benedek1 , M Bargetzi2 , M Hergersberg1 , A Huber1

1Zentrum für Labormedizin, Kantonsspital Aarau, 2Zentrum für Onkologie/Hämatologie und Transfusionsmedizin, Kantonsspital Aarau

Recently we published a proposal of a simpler, more practical approach for BCR-ABL fusion transcript quantification in a routine setting. We concluded that the major advantages are better standardization with the PAXgene Blood RNA System, use of only 2.5 ml peripheral blood (instead of 10-40 ml), no need to create a standard curve with each run and running samples as single measurements, i.e. non-repeated and estimating the inaccuracy of the method especially for low transcript rates. Using this proposed procedure should allow considerable savings in time and money, while preserving the level of accuracy. However, a major drawback is the reduced sensitivity of our approach.
Since our first proposal the results of more than 100 patient samples now clearly show the feasibility of our approach. Even with the reduced sensitivity of our approach we were able to amplify the BCR-ABL b3a2 MBCR transcript in all samples from CML patients treated with Imatinib mesylate. We conclude therefore, that our proposed RQ-PCR approach is a practical one for quantitative fusion transcript analysis not only in a research but also in a routine laboratory. It can be performed on peripheral blood samples and it is sensitive enough.

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