SULM – Schweizerische Union für Labormedizin | Union Suisse de Médecine de Laboratoire | Swiss Union of Laboratory Medicine

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S. Schneider1 , R. Groell1 , J. Muser1

1Klinische Chemie/Endokrinologie Kantonsspital Bruderholz, CH-4101 Bruderholz

Summary.
Homocysteine is a physiological amino acid and an intermediate in the degradation of proteins. Meanwhile homocysteine was also found to be an independent risk marker for cardiovascular events. An increasing number of requests have led us to implement a homocysteine method on our HP 1100 HPLC system. The application was installed and tested according to an verification plan to fulfil the requirements of the ISO/IEC 17025 accreditation protocols. The verification was performed with the homocysteine kit of Chromsystems on a HP 1100 HPLC system. The verification plan included the following tests: precision tests, linearity, method comparison (62 samples) and to write a standard operation procedure for the new method. The HPLC method was compared to the ABBOTT homocysteine immunoassay on the Axsym® and also to an external lab using the BAYER Centaur®. We present our data, statistical evaluations, SOP and show the successful introduction of the HPLC method in our routine lab.

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