SULM – Schweizerische Union für Labormedizin | Union Suisse de Médecine de Laboratoire | Swiss Union of Laboratory Medicine

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Soleiman Mahjoub1 , Mehein Zahraei2 , Fatemeh Karami3 , Mohammad-A Mohagheghi4 , Hojat Zeraeti5

1Dept. of Clinical Biochemistry, Babol Univ. of Medical Sciences, Babol, I.R.Iran, 2Dept. of Medical Biochemistry, Tehran Univ. of Medical Sciences, Tehran , I.R.Iran, 3Dept. of Clinical Biochemistry, Tarbiat Modarres University, Tehran, I.R.Iran, 4Institute of Cancer, Imam Khomaini Hospital, Tehran, I.R.Iran, 5Dept. of Epidemiology, Tehran Univ. of Medical Sciences, Tehran , I.R.Iran

OBJECTIVE:Mutation of the p53 gene leads to an accumulation of nonfanctional p53 protein in cell nucleus, which can be detected by immunohistochemical staining, this being largely due to the fact that such mutants have a markely longer half-life than the wild type p53 protein.MATERIALS & METHODS: We analyzed 31 patients with benign breast lesions and 82 patients with primary breast carcinoma. Tissue specimens were stained with H & E to determain the histopathological type and malignancy grade of tumors. The overexpression of p53 protein detected using immunohistochemical technique employing monoclonal antibody against the clone, DO-7 on all benign and malignant specimens. RESULTS: The p53 protein expression showed a significant difference between benign and malignant lesions. Within the group of benign lesions, 2 out of 31 (6.45%) had p53 expression >10% whereas 24 out of 82 (29.27%) malignant tumors demonstrated clearly positive stainig > 10% (P < 0.05). The p53 overexpression was not correlated with tumor type, tumor size, side of involved breast and nodal status(P>0.05), but there was a significant correlation with younger age (P < 0.05). There was a tendency for p53 overexpression with higer tumor grades and premonopausal status, although these did not achive significant (P>0.05). CONCLUSION: the lesions with benign morphology but having a p53 staining > 10%, may be attributed either to early molecular alteration of cancer that were histologicaly inapparent or to the presence of occult cancer cells.

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