SULM – Schweizerische Union für Labormedizin | Union Suisse de Médecine de Laboratoire | Swiss Union of Laboratory Medicine

Sie sind hier | Vous êtes ici:

min li 1 , xiaofei jiang 2 , qian gao 3 , ming guan 4 , yunxia gao 5 , yuan lu 6

1 Center of Laboratory Medicine, 12 Central Urumqi Road,Huashan Hospital, Fudan University, Shanghai, China, 2 Center of Laboratory Medicine, 12 Central Urumqi Road,Huashan Hospital, Fudan University, Shanghai, China, 3 Laboratory of Medical Molucular Virology, Shanghai Medical College, 120 Dongan Road,Fudan University, China, 4 Center of Laboratory Medicine, 12 Central Urumqi Road,Huashan Hospital, Fudan University, Shanghai, China, 5 Center of Laboratory Medicine,12 Central Urumqi Road, Huashan Hospital, Fudan University, Shanghai, China, 6 Center of Laboratory Medicine,12 Central Urumqi Road, Huashan Hospital, Fudan University, Shanghai, China

The most important virulence factor of S.epidermidis is considered to be biofilm formation. It is recently reported that agr regulates biofilm formation in S.epidermidis by regulation of AtlE(autolysin) and δ -toxin expression. There are many other important factors influencing the biofilm formation in S. epidermidis besides AtlE, such as Fbe (fibrinogen-binding protein), ClpP (ATP-dependent Clp protease proteolytic subunit). The regulatory effect of agr system on them is unknown. The aim of this study is to investigate which factors are regulated by agr system and to determine the regulatory effect of agr system on the biofilm formation in S.epidermidis. A proteomic analysis of S.epidermidis TüDagr (agr mutant) and S.epidermidis Tü3298 (wild type) were carried out. Differential levels of gene expression in S.epidermidis TüDagr and S.epidermidis Tü3298 were subsequently confirmed by quantitative real-time reverse transcription-PCR assay. The results showed that many proteins (16/19) were upregulated in agr mutant. They could be classified into four categories according to their function:(1) proteins for sugar metabolism (2) proteins for cell well biosynthesis (3) proteins for specific proteins biosynthesis (4) proteins with unknown function. Only three proteins showed a decreased synthesis. In agr mutant, ClpP was upregulated significantly, which is essential for biofilm formation. At the same time, D-alanine-D-alanine ligase also showed increased synthesis, which is important for cell well formation. However, the level of Fbe expression was equivalent to wild-type strain. Our data indicated that agr system regulates biofilm formation in S.epidermidis not only by regulation of AtlE and  δ -toxin expression, but also by regulation of ClpP protein expression. This was also the first report that agr could regulate cell well formation in S.epidermidis by regulation of D-alanine-D-alanine ligase expression.

Agenda

07. & 08.03.2019: Swiss eHealth Forum - Digitales Gesundheitswesen – Hope and > mehr

pipette

pipette 06/2018: Die Analyse der Darmflora | Analyse du microbiote

Aktuelle Ausgabe als E-Paper lesen

Jobs

17.12.2018: Cheflaboranten/in 80%, (HFR) Tafers