SULM – Schweizerische Union für Labormedizin | Union Suisse de Médecine de Laboratoire | Swiss Union of Laboratory Medicine

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Aslihan Avci1 , Erdinc Devrim1 , Imge Erguder1 , Nedim Karagenc1 , Ilker Durak1

1MD,Ankara University School of Medicine Department of Biochemistry

ADENOSINE DEAMINASE, XANTHINE OXIDASE ACTIVITIES AND NUCLEOTIDE LEVELS IN GASTRIC TISSUES FROM PATIENTS WITH GASTRIC CANCER
Objective: In this study, we aimed to measure levels of some nucleotides and activities of adenosine deaminase (ADA) and xanthine oxidase (XO) enzymes in cancerous and non cancerous human gastric tissues.
Methods: Ten gastric tissues with pathologically proven localized gastric cancer were used in the study. In the tissues, ADA and XO enzyme activities were measured by spectrophotometric method (1,2) and nucleotide levels (AMP, GMP) by High Performance Liquid Chromatography (HPLC) (3).
Results: No significant differences were established between AMP and GMP levels of the cancerous and non cancerous gastric tissues (3.11±1.92 vs. 2.51±1.59, p>0.05 and 4.06±1.68 vs. 2.86±2.04 nmol/mg, p>0.05, respectively). However, ADA activity was found to be lower (10.92±4.79 vs. 28.01±6.72 mIU/mg; p < 0.05), and XO activity higher in the cancerous tissues as compared with non cancerous ones (0.352±0.112 vs. 0.156±0.042 mIU/mg; p < 0.05).
Summary and Conclusion: Results suggest that decreased ADA and increased XO activities might be an attempt to lower salvage pathway synthesis of purine nucleotides, which may then lead to inhibition of cellular proliferation of cancerous tissue.
References:
1. Guisti G. Enzyme activities. In Bergmeyer UH, editor. Methods of enzymatic analysis. Weinheim Bergest: Verlag Chemia, 1974; p-1087-91
2. Hashimato S.A new spectrophotometric assay method of xanthine oxidase in crude tissue homogenate. Anal Biochem 1974; 62: 425-435
3. Keith F. Childs, Xue-Han Ning, Steven F. Bolling. Simultaneous detection of nucleotides, nucleosides and oxidative metabolites in myocardial biopsies. J Chromatography and Biomed Appl 1996; 678: 181-186

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