SULM – Schweizerische Union für Labormedizin | Union Suisse de Médecine de Laboratoire | Swiss Union of Laboratory Medicine

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Aslihan Avci1 , Recep Cetin2 , Erdinc Devrim1 , Bulent Kilicoglu3 , Ozden Candir4 , Ilker Durak1

1MD,Ankara University School of Medicine, Department of Biochemistry, Ankara-Turkey Ankara, 2MD,Oncology Education and Search Hospital, Department of General Surgery, Ankara-Turkey, 3MD,Ankara Education and Search Hospital, Department of General Surgery , Ankara-Turkey , 4MD,Süleyman Demirel University School of Medicine, Department of Pathology, Isparta-Turkey

Objective: In this study, we aimed to investigate possible molecular mechanism of cisplatin hepatotoxicity .
Methods: Fourteen Wistar Albino type female rats of 20 weeks old were used in the study. The rats weighed 220±15 g. Animals were maintained on a 12 hr light/dark cycle at room temperature (23±2°C) and allowed free access to food and water. The animals were divided into 2 groups randomly. Seven of them were used as control and the others were treated with a single dose of cisplatin i.p. (10 mg/kg body weight) Cisplatin was administered intraperitoneally (i.p.) in a single dose (10 mg/kg). In each group (control, cisplatin), there were 7 animals. Rats were sacrificed 72 h after the treatment. Then, the animals were sacrificed, the livers were removed and prepared for the biochemical and histopathological investigations (1). Oxidant (oxidation sensitivity, xanthine oxidase enzyme and malondialdehyde level) and antioxidant (superoxide dismutase, glutathione peroxidase and catalase enzymes) parameters were measured in liver tissues of the groups. Histopathological examination of the tissues was also performed.
Results: In the histopathological examination, sinusoidal congestion,hydropic and vacuolar degenerations, extensive disorganisation in hepatocytes in the liver tissues from cisplatin-treated animals. MDA level and XO activities were higher but CAT activity was lower in the cisplatin group compared with the control values.
Summary and Conclusion: Results suggest that oxidant stress may play part in the cisplatin-induced hepatotoxicity, and some agents with high antioxidant power may ameliorate this toxicity.
References:
1. Kavutcu M, Canbolat O, Öztürk S et al.(1996) Reduced enzymatic antioxidant defence mechanism in kidney tissues from gentamicin-treated guinea pigs, effects of vitamins E and C. Nephron 72:269-274

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