SULM – Schweizerische Union für Labormedizin | Union Suisse de Médecine de Laboratoire | Swiss Union of Laboratory Medicine

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Aslihan Avci1 , Recep Cetin2 , Erdinc Devrim1 , Imge Erguder1 , Bulent Kılınc3 , Ozden Candır4 , Ilker Durak1

1 MD,Ankara University School of Medicine, Department of Biochemistry, Ankara-Turkey , 2MD,Oncology Education and Search Hospital, Department of General Surgery, Ankara-Turkey, 3 MD, Ankara Education and Search Hospital, Department of General Surgery , Ankara-Turkey , 4MD,Süleyman Demirel University School of Medicine, Department of Pathology, Isparta-Turkey

Objectives:The aim of the present study was to investigate possible effects of methotrexate (MTX) on the oxidant/antioxidant status of liver tisuues from rats.
Methods: Ten Sprague-Dawley type female rats of 4 weeks old were used in the study. The rats weighed 200±15 g. Animals received humane care and the study protocol complied with the institution’s guidelines. MTX was given intravenous (60mg/m2/week) for 7 weeks. In each group (control, methotrexate), there were 5 animals. After the animals were sacrificed, their livers were removed and prepared for the biochemical investigations (1) Oxidant and antioxidant parameters were measured in liver tissues of the groups.
Results: There was significant oxidation in the liver tissues of methotrexate group. Although there were no meaningful differences between SOD (Superoxide dismutase), CAT (Catalase) activities and AOP (Antioxidant Potential) value of the grups, MDA (Malondialdehyde) was found to increase, and GSH-Px (Glutathion Peroxidase) decrease in the liver from MTX group.
Summary and Conclusion: Our results suggest that methotrexate causes oxidation in rat liver tissues. We suggest that oxidant stress may play part in the methotrexate-induced hepatotoxicity and some antioxidants, foods or vitamins may ameliorate this toxicity.


References:
1. Kavutcu M, Canbolat O, Öztürk S et al.(1996) Reduced enzymatic antioxidant defence mechanism in kidney tissues from gentamicin-treated guinea pigs, effects of vitamins E and C. Nephron 72:269-274

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