SULM – Schweizerische Union für Labormedizin | Union Suisse de Médecine de Laboratoire | Swiss Union of Laboratory Medicine

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HA Arab 1 , R Jamshidi2 , A Rasouli1 , M Hassanzadeh3

1 Department of Physiology, Pharmacology and Toxicology, Tehran University. Tehran, Iran , 2Department of Basic Sciences, College of Veterinary Medicine, University of Semnanm. Semnan, Iran, 3Department of Clinical Science, Faculty of Veterinary Medicine, Tehran University. Tehran, Iran

Increased metabolic activity, pulmonary hypertension and cardiac dysfunction are known as the most important features of chicken ascites syndrome. However, the causative agent of the syndrome is poorly understood. This study aimed to detect the generation of hydroxyl radicals in broiler chickens experiencing ascites. Ascites were induced in birds by administration of triiodothyrnine (T3) or reducing temperature. Different serological and biochemical parameters including hemoglobin (Hb), total red blood cell (RBC), hematochrit (PCV), ALT and AST enzymes as well as ratio of right ventricular weight to total ventricular weight (RV/TV) were measured to determine incidence of ascites. Salicylate hydroxylation method was used to detect the generation of hydroxyl radical in treated chickens. Hydroxylated metabolites of salicylate, namely 2,3- and 2,5-dihydroxy benzoic acids (DHBA), were measured by HPLC system as indices of hydroxyl radicals formation. Ascites syndrome occurred in groups received T3 or exposed to low temperature as it was judged by an increase in Hb, RBC, PCV, ALT, AST and RV/TV. The amounts of 2-3 and 2-5 DHBA were significantly (p < 0.001) increased in the chickens developed ascites compared to control group. It is suggesting that oxygen-derived hydroxyl radicals may be involved in the induction of chickens ascites syndrome.


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