SULM – Schweizerische Union für Labormedizin | Union Suisse de Médecine de Laboratoire | Swiss Union of Laboratory Medicine

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M. Ani, A.A. Moshtaghie, H. Ahmadvand

1Dept. Clin. Biochem, School of Pharmacy, Isfahan Univ. Med. Sci. Isfahan. IRAN, 2 , 3

Introduction: The importance of LDL oxida-tion as causal factor in atherosclerosis is the subject of widespread investigation. Oxidative modification of LDL is responsible for accumulating and forming foam cells promoting their residence in the endothelium and leading to atherosclerotic fatty streak lesions. There are some evidence that metal ions may affect the degree of LDL oxidation both in vivo and in vitro. Thus metal ions that are used in medicine and industry has attracted the attention of many researchers. Titamium salts are widely used in industry, in pharmacy for tablet coating and making chemical sunscreens and medicine as photocatalysts with bacteriocidal activity. In this study the effect of titanium on lipid peroxidation was investigated.

Methods: Groups of male Wistar rats were administered(i.p.) with different doses of titanium chloride; 2.5 mg/kg for 10 days (acute), and 0.75 mg/kg for 30 and/or 60 days (chronic). Blood samples were then collected and the degree of lipid peroxidation was determined using TBA method. Purified LDL was also incubated for 24 hr in the presence of 10 uM Ti. The same experiments were performed with either of Cu, Fe and V, other oxidizing elements, for comparison. LDL oxidation was measured by comparing the changes in absorbance at 234 nm, related to conjugated dien production, in the medium at 0 and 24 hr post-incubation.

Results: It was shown that the presence of Ti resulted in extensive oxidative modification of LDL. Thus, acute dose of titanium increased lipid peroxidation by 65%, whereas chronic treatment of the animals for 30 and 60 days resulted in increase of 19 and 30% respectively. Ti as well as V, Fe and Cu in vitro also led to increased LDL oxidation by 15, 20, 38 and 42% respectively.

Conclusion: Data presented here indicate that titanium is affective in lipid peroxidation and so it may exert some atherogenic activities which should be considered serioushy in people exposed to this metal ion.


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