SULM – Schweizerische Union für Labormedizin | Union Suisse de Médecine de Laboratoire | Swiss Union of Laboratory Medicine

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B Alani 1 , N Zarghami 2 , S samadzadeh 3 , H Fani 4

1Dept. Clinical Biochemistry and RIA. Drug Applied Research Center, Tabriz University of Medical Sciences, 2Dept. Clinical Biochemistry and RIA. Drug Applied Research Center, Tabriz University of Medical Sciences, 3Dept. Urology,Urmia University of Medical Sciences, 4Dept. Urology,Urmia University of Medical Sciences

Acute rejection of renal transplant is a serious systemic inflammatory disorder, which is resulted in decreased half-life of transplant organ, and this is diagnosed by invasive biopsy procedure on kidney. Therefore, applying a novel and noninvasive diagnostic procedure in acute renal transplantation could be able to improve survival of transplanted tissue. 32 patients, which underwent to kidney transplantation from may to august 2003 in teaching imam hospital, Urmia, Iran, were studied. From each patient 5 different samples, day 2 to day 20 after renal transplantation, were continuously obtained and the serum levels of granzyme B enzyme were measured by immunoassay method. Patients were followed up 9 to 12 months for status of transplanted tissue. It was shown that 13 patients (40.5 %) with mean age of 33 years and duration of dialysis with 18 months had stable function of transplanted tissue. The mean serum levels of granzyme B in these patients were less than 148 U/ mL 19 patients (59.9 %) had unstable function of transplanted tissue which in 6 patients (19%) acute rejection and in 13 patients other etiologies were involved. The mean age of patients were 43.74 years and mean of duration of dialysis were 27 months. The mean serum levels of granzyme B in acute rejection in first sample (day 2-5) 250.20 +/- 46.25 u/mL, in second sample (day 6-8) 286.50 +/- 49.92 u/mL, in third sample (day 9-11) 275.60 +/- 68.40 u/mL, and in forth sample (day 12-16) 253.17 +/- 86.14 u/mL were respectively and in relation to other two group, the difference was significant (p < 0.05). In fifth sample (day 17-20), the mean serum levels of granzyme B in acute rejection group were 158.17 +/- 29 u/mL and there was no statistically significant between them. It was speculated that continuous measurement of granzyme B enzayme in whole blood by immunoassay methods in three weeks after renal transplantation could be a rapid and noninvasive diagnostic procedure for renal transplant rejection.

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